Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A 2in1 Cloning System Enables Ratiometric Bimolecular Fluorescence Complementation (rBiFC)

1 Nov 2012

Abstract excerpt

Gene expression and binary interaction techniques are vital tools that shape our understanding of protein complexes. An inherent flaw, however, with most current protein-protein interaction techniques is the variability in expression levels for fusion proteins when using several individual plasmids. Here, we describe a novel recombination-based cloning strategy called 2in1 that enables co-expression of fusion...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biotin and Related Studies
Share to Monoclonal and Polyclonal Antibodies Research
Share to Viral Infectious Diseases and Gene Expression in Insects

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 23066669
DOI: 10.2144/000113941

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A 2in1 Cloning System Enables Ratiometric Bimolecular Fluorescence Complementation (rBiFC)PMID 23066669Read ↗

<strong>BiFC Magic: Shedding Light on Protein-Protein Interactions</strong>Semantic proximity Read ↗

Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions.Semantic proximity Read ↗

Chimeric Autofluorescent Proteins as Photophysical Model System for Multicolor Bimolecular Fluorescence Complementation.Semantic proximity Read ↗

Firefly luciferase complementation imaging assay for protein-protein interactions in plants.Semantic proximity Read ↗

Visualizing interactions along the Escherichia coli twin-arginine translocation pathway using protein fragment complementation.Semantic proximity Read ↗

Barcode Fusion Genetics-Protein-fragment Complementation Assay (BFG-PCA): tools and resources that expand the potential for binary protein interaction discoverySemantic proximity Read ↗

Multigene expression of protein complexes by iterative modification of genomic Bacmid DNASemantic proximity Read ↗

Reverse PCA, a systematic approach for identifying genes important for the physical interaction between protein pairs.Semantic proximity Read ↗

Detection of plum pox potyviral protein-protein interactions in planta using an optimized mRFP-based bimolecular fluorescence complementation system.Semantic proximity Read ↗

Analysis of Protein-Protein Interactions by Split Luciferase Complementation Assay.Semantic proximity Read ↗

In situ dimerization of multiple wild type and mutant zinc transporters in live cells using bimolecular fluorescence complementation.Semantic proximity Read ↗

Dimerization of ABCG2 analysed by bimolecular fluorescence complementation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.