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Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA

2 Sept 2009

Abstract excerpt

BACKGROUND: Many cellular multi-protein complexes are naturally present in cells at low abundance. Baculovirus expression offers one approach to produce milligram quantities of correctly folded and processed eukaryotic protein complexes. However, current strategies suffer from the need to produce large transfer vectors, and the use of repeated promoter sequences in baculovirus, which itself produces proteins that...

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PMID: 19725957
DOI: 10.1186/1471-2199-10-87

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