Article
Firefly luciferase complementation imaging assay for protein-protein interactions in plants.
Plant physiology - 1 Feb 2008
Chen Huamin, Zou Yan, Shang Yulei, Lin Huiqiong, Wang Yujing, Cai Run, Tang Xiaoyan, Zhou Jian-Min
Abstract excerpt
The development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. The previously described techniques, fluorescence resonance energy transfer and bimolecular fluorescence complementation, which are used widely for protein-protein interaction studies in plants, require extensive instrumentation. To facilitate protein-protein...
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