Article
Visualizing interactions along the Escherichia coli twin-arginine translocation pathway using protein fragment complementation.
PloS one - 16 Feb 2010
Kostecki Jan S, Li Haiming, Turner Raymond J, DeLisa Matthew P
Abstract excerpt
The twin-arginine translocation (Tat) pathway is well known for its ability to export fully folded substrate proteins out of the cytoplasm of gram-negative and gram-positive bacteria. Studies of this mechanism in Escherichia coli have identified numerous transient protein-protein interactions that guide export-competent proteins through the Tat pathway. To visualize these interactions, we have adapted bimolecular...
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