Article
Chimeric Autofluorescent Proteins as Photophysical Model System for Multicolor Bimolecular Fluorescence Complementation.
The journal of physical chemistry. B - 23 Mar 2017
Peter Sébastien, Oven-Krockhaus Sven Zur, Veerabagu Manikandan, Rodado Virtudes Mira, Berendzen Kenneth W, Meixner Alfred J, Harter Klaus, Schleifenbaum Frank E
Abstract excerpt
The yellow fluorescent protein (YFP) is frequently used in a protein complementation assay called bimolecular fluorescence complementation (BiFC), and is employed to visualize protein-protein interactions. In this analysis, two different, nonfluorescent fragments of YFP are genetically attached to proteins of interest. Upon interaction of these proteins, the YFP fragments are brought into proximity close enough...
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