Article
AFM-imaging diagnosis method for single nucleotide polymorphism using molecular beacon DNA as an intramolecular ligation template of target DNA and a viewable indicator.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Jan 2012
Yoshinaga Hisao, Nakano Koji, Soh Nobuaki, Imato Toshihiko
Abstract excerpt
An AFM-imaging-based method for single nucleotide polymorphism (SNP) analysis is described. A stem-loop-forming 34-mer oligonucleotide (p34s) was designed. p34s contains the complementary sequence for K-ras (5'-GGT GGC-3', t6G), one of the human oncogenes, at the 5'-end for target-recognition and five successive phosphorothioate linkages in the loop. The functional probe, either alone or hybridized with target...
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