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Article

Preparation and use of metal surface-immobilized DNA hairpins for the detection of oligonucleotides.

Nature protocols - 1 Jan 2007

Strohsahl Christopher M, Miller Benjamin L, Krauss Todd D

Abstract excerpt

The following protocol describes the experimental steps used to prepare arrayable and label-free biological sensors that are based on the fluorescence unquenching of DNA hairpins immobilized on metal surfaces. This two-part protocol describes both the creation of gold-coated substrates and the oligonucleotide surface self-assembly process that transforms the substrates into reporters for detecting DNA. Using this...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17853865
DOI: 10.1038/nprot.2007.301

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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