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Article

A universal and label-free impedimetric biosensing platform for discrimination of single nucleotide substitutions in long nucleic acid strands.

Biosensors & bioelectronics - 30 Jun 2018

Mills Dawn M, Martin Christopher P, Armas Stephanie M, Calvo-Marzal Percy, Kolpashchikov Dmitry M, Chumbimuni-Torres Karin Y

Abstract excerpt

We report a label-free universal biosensing platform for highly selective detection of long nucleic acid strands. The sensor consists of an electrode-immobilized universal stem-loop (USL) probe and two adaptor strands that form a 4J structure in the presence of a specific DNA/RNA analyte. The sensor was characterized by electrochemical impedance spectroscopy (EIS) using K3[Fe(CN)6]/K4[Fe(CN)6] redox couple in...

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PMID: 29524915
DOI: 10.1016/j.bios.2018.02.059

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