Article
Metabotyping of the C. elegans sir-2.1 mutant using in vivo labeling and (13)C-heteronuclear multidimensional NMR metabolomics.
ACS chemical biology - 21 Dec 2012
An Yong Jin, Xu Wen Jun, Jin Xing, Wen He, Kim Hyesook, Lee Junho, Park Sunghyouk
Abstract excerpt
The roles of sir-2.1 in C. elegans lifespan extension have been subjects of recent public and academic debates. We applied an efficient workflow for in vivo(13)C-labeling of C. elegans and (13)C-heteronuclear NMR metabolomics to characterizing the metabolic phenotypes of the sir-2.1 mutant. Our method delivered sensitivity 2 orders of magnitude higher than that of the unlabeled approach, enabling 2D and 3D NMR...
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