Article
A low-cost hydrogel chip for SNP typing by the incorporation of Cy5-dCTP into label-free allele-specific probes hybridizing to gel-immobilized targets.
Journal of nanoscience and nanotechnology - 1 Sept 2012
Wang Hui, Chen Zhiyao, Fu Xiaoying, Li Youzhi, Tang Xiaowen, Wu Haiping, Zou Bingjie, Zhou Guohua
Abstract excerpt
A 3-dimensional (3-D) polyacrylamide gel microarray based on dual-color fluorescence hybridization was an efficient SNP typing method with a high-throughput, but it is expensive to use dual dye-labeled allele-specific probes to type various SNPs. To lower the typing cost on 3-D polyacrylamide gel microarray, we propose a novel method by incorporating Cy5-dCTP into label-free allele-specific probes hybridizing to...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.