Article
Detection of multiple SNPs in numerous samples with polyacrylamide gel-based microarray.
Journal of nanoscience and nanotechnology - 1 Jan 2010
Cheng Lu, Sun Beili, Sun Yu, Xiao Pengfeng, Ge Qinyu, Zheng Yuanzhuo, Ke Xiaoyan, Zhou Ying, Zhang Minghao, Chen Ping, Lu Zuhong
Abstract excerpt
Previously we have developed polyacrylamide gel-based DNA microarray to genotype single nucleotide polymorphism (SNP) in a large number of samples, which has been proved as a simple and robust platform for high-throughput SNP screening. Here we improved this method to detect multiple SNPs by introducing multiplex polymerase chain reaction (multiplex-PCR) and immobilizing the products of multiplex-PCR to fabricate...
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