Article
A gel-based solid-phase amplification and its application for SNP typing and sequencing on-chip.
The Analyst - 1 Dec 2009
Huang Huan, Xiao Pengfeng, Qi Zongtai, Bu Ying, Liu Wenbo, Zhou Guohua
Abstract excerpt
As conventional solid-phase amplification (SPA) on a two-dimensional slide has a low amplification capacity due to a limited amount of immobilized primers, we propose a three-dimensional SPA by immobilizing primers in hydrogel attached to a slide. One of the PCR primers, modified with an acrylami...
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