Article
Fabrication of 3-D gel microarrays directly with raw polymerase chain reaction products by heat-directed polymerization.
Electrophoresis - 1 Jun 2008
Pan Zhiqiang, Li Yanqiang, Shan Qun, Hong Xiaohong, Zhou Dongrui, Zhang Minghao, Chen Ping, Xiao Pengfeng, Lu Zuhong
Abstract excerpt
In this study, a new method for fabricating 3-D polyacrylamide gel microarrays was presented. In the method, a 3-D gel microarray was successfully fabricated by directly heating the slides with arrayed prepolymer at 80 degrees C for 2 min. The prepolymer on the microarray only contained 40% v/v raw/unpurified PCR products, 1.4% ammonium persulfate (APS), 6% acrylamide, and 20% glycerol and the balance H2O but no...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.