Article
Single-nucleotide polymorphism typing based on pyrosequencing chemistry and acryl-modified glass chip.
Electrophoresis - 1 Mar 2009
Huang Huan, Wu Haiping, Xiao Pengfeng, Zhou Guohua
Abstract excerpt
A new method (termed as "chip-BAMPER" (bioluminometric assay coupled with modified primer extension reactions)) for single-nucleotide polymorphism (SNPs) genotyping was developed by pyrosequencing chemistry coupled with hydrogel chip immobilized with single-stranded target DNAs. The method is based on allele-specific extension reaction, which is switched by the base type in the 3' end of allele-specific primers....
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