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Article

Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase.

Nucleic acids research - 6 Sept 2017

Agudo Rubén, Calvo Patricia A, Martínez-Jiménez María I, Blanco Luis

Abstract excerpt

We have developed a straightforward fluorometric assay to measure primase-polymerase activity of human PrimPol (HsPrimPol). The sensitivity of this procedure uncovered a novel RNA-dependent DNA priming-polymerization activity (RdDP) of this enzyme. In an attempt to enhance HsPrimPol RdDP activity, we constructed a smart mutant library guided by prior sequence-function analysis, and tested this library in an...

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Identifiers

PMID: 28911121
DOI: 10.1093/nar/gkx633
PMCID: PMC5587808

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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