Article
Positioning of CCA-arms of the A- and the P-tRNAs towards the 28S rRNA in the human ribosome.
Biochimie - 1 Feb 2013
Bulygin Konstantin, Malygin Alexey, Hountondji Codjo, Graifer Dmitri, Karpova Galina
Abstract excerpt
Nucleotides of 28S rRNA involved in binding of the human 80S ribosome with acceptor ends of the A site and the P site tRNAs were determined using two complementary approaches, namely, cross-linking with application of tRNA(Asp) analogues substituted with 4-thiouridine in position 75 or 76 and hydroxyl radical footprinting with the use of the full sized tRNA and the tRNA deprived of the 3'-terminal trinucleotide...
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