Article
Specificity of spontaneous mutation in the lacI gene cloned into bacteriophage M13.
Mutation research - 1 Jan 1990
Yatagai F, Glickman B W
Abstract excerpt
We have studied the specificity of spontaneous mutation in the lacI gene of Escherichia coli cloned into bacteriophage M13. The comparison of the spectrum of 85 spontaneous mutations with that of the lacI gene carried on an E. coli F' episone revealed the following characteristics: (i) base substitution was predominant, accounting for 80% of spontaneous events compared with only 11% on the F' episome; (ii) among...
Topics
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Coliphages
- DNA, Single-Stranded
- Genes, Bacterial
- Lac Operon
- Molecular Sequence Data
- Mutation
- Repressor Proteins
- Transcription Factors