Article
Identification of mutation sites of a temperature-sensitive mutant of HCMV DNA polymerase activity.
Archives of virology - 1 Jan 1994
Ihara S, Takekoshi M, Mori N, Sakuma S, Hashimoto J, Watanabe Y
Abstract excerpt
The human cytomegalovirus (strain Towne) temperature-sensitive mutant ts 256 exhibits a virus specific DNA polymerase-negative phenotype. The position of the mutation of ts 256 was determined by three step marker-rescue assays to be within a 5.1 kb XbaI-BamHI fragment between map unit 0.33 and 0.35 in a HindIII-D fragment located in a long unique region. Nucleotide sequencing showed that the 5.1 kb fragment...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Line
- Conserved Sequence
- Cosmids
- Cytomegalovirus
- DNA-Directed DNA Polymerase
- Humans
- Molecular Sequence Data
- Mutation
- Restriction Mapping