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Article

Measurement of diversification in the immunoglobulin light chain gene of DT40 cells.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Sale Julian E

Abstract excerpt

The immunoglobulin loci of the genetically tractable chicken B cell line DT40 provide a unique opportunity to study the cellular response to endogenously generated DNA damage in a chromosomal context. Abasic sites generated by the concerted action of Activation-Induced Deaminase (AID) and Uracil DNA Glycosylase result in both homologous recombination-dependent gene conversion and translesion synthesis-dependent...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 22941620
DOI: 10.1007/978-1-61779-998-3_29

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Measurement of diversification in the immunoglobulin light chain gene of DT40 cells.PMID 22941620Read ↗

Immunoglobulin gene conversion in chicken DT40 cells largely proceeds through an abasic site intermediate generated by excision of the uracil produced by AID‐mediated deoxycytidine deaminationSemantic proximity Read ↗

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Establishment of the DNA repair-defective mutants in DT40 cells.Semantic proximity Read ↗

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Activation-induced cytidine deaminase-mediated hypermutation in the DT40 cell line.Semantic proximity Read ↗

AID-mediated diversification within the IgL locus of chicken DT40 cells is restricted to the transcribed IgL gene.Semantic proximity Read ↗

Understanding the immunoglobulin locus specificity of hypermutation.Semantic proximity Read ↗

DNA polymerases nu and theta are required for efficient immunoglobulin V gene diversification in chicken.Semantic proximity Read ↗

Requirement of the activation-induced deaminase (AID) gene for immunoglobulin gene conversion.Semantic proximity Read ↗

Activation-induced cytidine deaminase initiates immunoglobulin gene conversion and hypermutation by a common intermediate.Semantic proximity Read ↗

Ablation of XRCC2/3 transforms immunoglobulin V gene conversion into somatic hypermutation.Semantic proximity Read ↗

Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40.Semantic proximity Read ↗

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