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Article

Competitive assay for theophylline based on an abasic site-containing DNA duplex aptamer and a fluorescent ligand.

Chemistry (Weinheim an der Bergstrasse, Germany) - 1 Oct 2012

Sato Yusuke, Zhang Yushuang, Nishizawa Seiichi, Seino Takehiro, Nakamura Kodai, Li Minjie, Teramae Norio

Abstract excerpt

A fluorescence assay for theophylline, one of the common drugs for acute and chronic asthmatic conditions, has been developed based on an abasic site-containing DNA duplex aptamer (AP aptamer) in combination with an abasic site-binding fluorescent ligand, riboflavin. The assay is based on the competitive binding of theophylline and riboflavin at the abasic (AP) site of the AP aptamer. In the absence of...

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PMID: 22915350
DOI: 10.1002/chem.201201298

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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