Article
Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domains.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Singh Purnima, Szabó Piroska E
Abstract excerpt
Imprinted genes are marked by parental allele specific DNA methylation and histone modifications which regulate their monoallelic expression. Chromatin immunoprecipitation (ChIP) is the technique of choice to characterize the histones associated with either maternal or paternal chromosomes. To study allele-specific chromatin composition at imprinted regions, the method has to be efficient to work on limiting...
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