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[Mutation scanning of short amplicons: optimization of DNA melting conditions].

Molekuliarnaia biologiia - 1 Jan 2000

Botezatu I V, Zhordania K I, Karseladze A I, Stroganova A M, Kondratova B N, Shelepov V P, Telkov M V, Likhtenshtein A V

Abstract excerpt

High resolution melting analysis (HRMA) using special "saturating" fluorescent dyes is a new and very effective approach to genotyping and mutation scanning. HRMA, which is carried out usually just after PCR without any intermediate manipulations (the "closed tube" format), is simple and high-throughput method excluding sample cross-contaminations. The "closed tube" format makes, however, HRMA dependent on PCR...

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PMID: 22888635

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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