Article
[Scanning for KRAS, NRAS, BRAF, and PIK3CA mutations by DNA melting analysis with TaqMan probes].
Molekuliarnaia biologiia - 1 Jan 2000
Botezatu I V, Panchuk I O, Stroganova A M, Senderovich A I, Kondratova V N, Shelepov V P, Lichtenstein A V
Abstract excerpt
Scanning for mutations by DNA melting analysis (DMA) is based on asymmetric PCR followed by the melting of duplexes formed by single-stranded amplicons with TaqMan probes. The method is optimally suited for clinical genetic testing; it is easy to perform, high-throughput, and sensitive. The detection limit of mutant alleles by the DMA method is about 3%, which is much higher than the sensitivity of Sanger...
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