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Article

DNA melting analysis: application of the "open tube" format for detection of mutant KRAS.

Analytical biochemistry - 15 Dec 2011

Botezatu Irina V, Kondratova Valentina N, Shelepov Valery P, Lichtenstein Anatoly V

Abstract excerpt

High-resolution melting (HRM) analysis is a very effective method for genotyping and mutation scanning that is usually performed just after PCR amplification (the "closed tube" format). Though simple and convenient, the closed tube format makes the HRM dependent on the PCR mix, not generally optimal for DNA melting analysis. Here, the "open tube" format, namely the post-PCR optimization procedure (amplicon...

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Source

Identifiers

PMID: 21889482
DOI: 10.1016/j.ab.2011.08.015

Genes

KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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