Article
PIK3CA hotspot mutation scanning by a novel and highly sensitive high-resolution small amplicon melting analysis method.
The Journal of molecular diagnostics : JMD - 1 Sept 2010
Vorkas Panagiotis A, Poumpouridou Nikoleta, Agelaki Sophia, Kroupis Christos, Georgoulias Vassilis, Lianidou Evi S
Abstract excerpt
Somatic mutations in the PIK3CA gene have been discovered in many human cancers, and their presence correlates to therapy response. Three "hotspot" mutations within the PIK3CA gene are localized in exons 9 and 20. High-resolution melting analysis (HRMA) is a highly sensitive, robust, rapid, and cost-effective mutation analysis technique. We developed a novel methodology for the detection of hotspot mutations in...
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