Article
pH effects on binding between the anthrax protective antigen and the host cellular receptor CMG2.
Protein science : a publication of the Protein Society - 1 Oct 2012
Rajapaksha Maheshinie, Lovell Scott, Janowiak Blythe E, Andra Kiran K, Battaile Kevin P, Bann James G
Abstract excerpt
The anthrax protective antigen (PA) binds to the host cellular receptor capillary morphogenesis protein 2 (CMG2) with high affinity. To gain a better understanding of how pH may affect binding to the receptor, we have investigated the kinetics of binding as a function of pH to the full-length monomeric PA and to two variants: a 2-fluorohistidine-labeled PA (2-FHisPA), which is ∼1 pH unit more stable to variations...
Topics
- Antigens, Bacterial
- Bacterial Toxins
- Crystallography, X-Ray
- Fluorescence Resonance Energy Transfer
- Humans
- Hydrogen-Ion Concentration
- Kinetics
- Linear Models
- Membrane Proteins
- Models, Molecular
- Mutation
- Protein Binding
- Receptors, Peptide