Site specific radioiodination of recombinant hirudin.

doi:10.1016/0003-2697(90)90105-i

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Article

Site specific radioiodination of recombinant hirudin.

Analytical biochemistry - 1 Sept 1990

Tuong A, Maftouh M, Picard C, Gachon M

Abstract excerpt

Recombinant hirudin variant rHV2-Lys47 was radioiodinated using the chloramine-T method. Depending on the reaction pH, the two tyrosine residues, Tyr3 and Tyr63, responded differently to iodination but without change in total iodination yield. Of the incorporated -125 iodine 80% was located on Ty...

Topics

Amino Acid Sequence
Carboxypeptidases
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Genetic Variation
Hirudins
Hydrogen-Ion Concentration
Iodine
Iodine Radioisotopes
Molecular Sequence Data
Recombinant Proteins

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