Article
Anti-hirudin monoclonal antibodies directed toward discontinuous epitopes of the hirudin amino-terminal and epitopes involving the carboxy-terminal hirudin amino acids.
Analytical biochemistry - 1 Oct 1993
Koch C, Whitechurch O, Cordier P, Roitsch C
Abstract excerpt
A panel of eight monoclonal antibodies (MAbs) was obtained against recombinant hirudin variant 2 (rHV2). Specificities of the eight MAbs indicate that four of them recognize C-terminal amino acid residues (Group A) and four are directed against discontinuous epitopes and recognize a determinant (...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Chromatography, Affinity
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- Diphtheria Toxoid
- Epitopes
- Female
- Genetic Variation
- Hirudins
- Immunoenzyme Techniques
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Recombinant Proteins
- Saccharomyces cerevisiae