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Article

Distinct binding sites of Ala48-hirudin1-47 and Ala48-hirudin48-65 on alpha-thrombin.

The Journal of biological chemistry - 15 Jan 1990

Dodt J, Köhler S, Schmitz T, Wilhelm B

Abstract excerpt

The interaction of alpha-thrombin with Ala48-hirudin, Ala48-hirudin1-47, and Ala48-hirudin48-65 was analyzed. Mutations at Pro48 were found to cause only slight changes in the kon (human: 3.1 +/- 0.3 x 10(8) M-1 s-1; bovine: 1.03 +/- 0.3 x 10(8) M-1 s-1) and koff (human: 0.4 +/- 0.2 x 10(-3) s-1;...

Topics

Alanine
Amino Acid Sequence
Animals
Cattle
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Hirudins
Humans
Molecular Sequence Data
Mutation
Proline
Thrombin

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PMID: 2295615

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2295615-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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