Article
Use of fragments of hirudin to investigate thrombin-hirudin interaction.
European journal of biochemistry - 22 Feb 1990
Dennis S, Wallace A, Hofsteenge J, Stone S R
Abstract excerpt
Site-directed mutagenesis was used to create hirudin in which Asn52 was replaced by methionine. Cyanogen bromide cleavage at this unique methionine resulted in two fragments. These fragments have been used to study the kinetic mechanism of the inhibition of thrombin by hirudin and to identify are...
Topics
- Antithrombin III
- Asparagine
- Binding Sites
- Binding, Competitive
- Escherichia coli
- Hirudins
- Humans
- Kinetics
- Methionine
- Mutation
- Pancreatic Elastase
- Peptide Fragments
- Protein Binding
- Protein Engineering
- Recombinant Proteins
- Thrombin
- Trypsin