Article
Probing the distance between the two binding sites of hirudin for its interaction with the active site and the fibrin(ogen)-binding site of alpha-thrombin.
Biomedica biochimica acta - 1 Jan 1991
Wagner U, Schmitz T, Otte M, Dodt J
Abstract excerpt
The interaction of delta (Ser50)-hirudin with alpha-thrombin has been investigated. Deletion of Ser50 of r-hirudin caused a 2.7 fold increase of the Ki for its complex with alpha-thrombin. Determination of the rate constants kon and koff for complex formation showed that this effect was mainly due to a change in koff.
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Fibrin
- Fibrinogen
- Hirudins
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Oligopeptides
- Thrombin