Recombinant hirudin: kinetic mechanism for the inhibition of human thrombin.

doi:10.1093/protein/4.3.295

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Article

Recombinant hirudin: kinetic mechanism for the inhibition of human thrombin.

Protein engineering - 1 Feb 1991

Stone S R, Hofsteenge J

Abstract excerpt

Recombinant hirudin variant-2(Lys47), was found to be a competitive inhibitor of human alpha-thrombin with respect to peptidyl p-nitroanilide substrates. These results contrast with those of Degryse and coworkers that suggest that recombinant hirudin variant-2(Lys47) inhibited thrombin by a nonco...

Topics

Amino Acid Sequence
Binding Sites
Binding, Competitive
Hirudins
Humans
Kinetics
Models, Molecular
Molecular Sequence Data
Mutation
Recombinant Proteins
Thrombin

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