Characterization of the deamidated forms of recombinant hirudin.

doi:10.1021/bi00150a024

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Article

Characterization of the deamidated forms of recombinant hirudin.

Biochemistry - 8 Sept 1992

Tuong A, Maftouh M, Ponthus C, Whitechurch O, Roitsch C, Picard C

Abstract excerpt

Recombinant hirudin variant rHV2-Lys 47 (MW = 6906.5) was intentionally deamidated by incubation in pH 9 phosphate buffer at 37 degrees C. Anion-exchange HPLC analysis showed that 11 forms could be generated. These were isolated and purified by combined anion-exchange and reversed-phase HPLC. Aci...

Topics

Amino Acid Sequence
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Cloning, Molecular
Genetic Variation
Hirudins
Indicators and Reagents
Mass Spectrometry
Molecular Sequence Data
Protein Conformation
Recombinant Proteins
Saccharomyces cerevisiae

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