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Article

Characterization of the deamidated forms of recombinant hirudin.

Biochemistry - 8 Sept 1992

Tuong A, Maftouh M, Ponthus C, Whitechurch O, Roitsch C, Picard C

Abstract excerpt

Recombinant hirudin variant rHV2-Lys 47 (MW = 6906.5) was intentionally deamidated by incubation in pH 9 phosphate buffer at 37 degrees C. Anion-exchange HPLC analysis showed that 11 forms could be generated. These were isolated and purified by combined anion-exchange and reversed-phase HPLC. Acid-catalyzed carboxyl methylation was used to introduce a mass shift of +15 amu per deamidated residue present in the...

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Source

Identifiers

PMID: 1525166
DOI: 10.1021/bi00150a024

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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