Intrinsic fluorescence changes and rapid kinetics of the reaction of thrombin with hirudin.

Article

Intrinsic fluorescence changes and rapid kinetics of the reaction of thrombin with hirudin.

The Journal of biological chemistry - 5 Aug 1992

Jackman M P, Parry M A, Hofsteenge J, Stone S R

Abstract excerpt

Stopped-flow fluorescence spectroscopy has been used to study the reaction of human alpha-thrombin with recombinant hirudin variant 1 (rhir) at 37 degrees C and an ionic strength of 0.125 M. A 35% enhancement in intrinsic fluorescence accompanied formation of the thrombin-rhir complex. Over one t...

Topics

Genetic Variation
Hirudins
Humans
Kinetics
Mathematics
Models, Theoretical
Peptide Fragments
Protein Conformation
Recombinant Proteins
Spectrometry, Fluorescence
Thrombin

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