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Article

Validation and comparison of different PCR-based methods for detection of hepatitis B virus precore region mutants.

Journal of virological methods - 1 Oct 2005

Flichman D, Maina A, Colombatto P, Bonino F, Brunetto M R

Abstract excerpt

Hepatitis virus variants detection is useful in clinical practice; however, methods that are used for their identification may influence the results significantly. Three PCR-based assays for quantitation of G1896A precore HBV mutants: two allele specific PCRs, single tube (single-AS-PCR) with enzymatic restriction or separate tubes (twin-AS-PCR) and one oligohybridization assay (OA) with three probes were...

Topics

DNA, Viral
Hepatitis B Core Antigens
Hepatitis B virus
Humans
Mutation
Polymerase Chain Reaction
Sensitivity and Specificity

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 15993954
DOI: 10.1016/j.jviromet.2005.05.007

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