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Article

Development of novel triplex single-step real-time PCR assay for detection of Hepatitis Virus B and C simultaneously.

Virology - 1 May 2016

Prakash Shantanu, Jain Amita, Jain Bhawana

Abstract excerpt

Multiplex RT-PCR assays are widely used tools for detection of hepatitis viruses, but none of them provide quality check of sample. In the present study we developed a single-step triplex real-time polymerase chain reaction (PCR) assay for detection of Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) with sample quality check, by using β-actin as housekeeping gene. The primers and probes were self-designed and...

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PMID: 26914508
DOI: 10.1016/j.virol.2016.01.029

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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