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Article

Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos.

Proceedings of the National Academy of Sciences of the United States of America - 3 Jul 2012

Ochiai Hiroshi, Sakamoto Naoaki, Fujita Kazumasa, Nishikawa Masatoshi, Suzuki Ken-ichi, Matsuura Shinya, Miyamoto Tatsuo, Sakuma Tetsushi, Shibata Tatsuo, Yamamoto Takashi

Abstract excerpt

To understand complex biological systems, such as the development of multicellular organisms, it is important to characterize the gene expression dynamics. However, there is currently no universal technique for targeted insertion of reporter genes and quantitative imaging in multicellular model systems. Recently, genome editing using zinc-finger nucleases (ZFNs) has been reported in several models. ZFNs consist...

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PMID: 22711830
DOI: 10.1073/pnas.1202768109
PMCID: PMC3390856

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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