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Article

Molecular analysis of hprt mutants induced by 2-cyanoethylene oxide in human lymphoblastoid cells.

Mutation research - 1 Nov 1990

Recio L, Simpson D, Cochrane J, Liber H, Skopek T R

Abstract excerpt

The mutagenic epoxide metabolite of acrylonitrile, 2-cyanoethylene oxide (ANO), was used to treat human TK6 lymphoblasts (150 microM x 2 h ANO). A collection of hypoxanthine-phosphoribosyltransferase (hprt) mutants was isolated and characterized by dideoxy sequencing of cloned hprt cDNA. Base-pair substitution mutations in the hprt coding region were observed in 19/39 of hprt mutants: 11 occurred at AT base pairs...

Topics

Base Composition
Base Sequence
Blotting, Southern
Carcinogens
Cell Line
Chromosome Deletion
Cloning, Molecular
Ethylene Oxide
Exons
Humans
Hypoxanthine Phosphoribosyltransferase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutagenicity Tests

Mutation

Polymerase Chain Reaction

Source

Identifiers

PMID: 2270092
DOI: 10.1016/0165-1218(90)90085-g

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2270092-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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