Article
Analysis of hprt mutations occurring in human TK6 lymphoblastoid cells following exposure to 1,2,3,4-diepoxybutane.
Mutagenesis - 1 Mar 1997
Steen A M, Meyer K G, Recio L
Abstract excerpt
1,3-Butadiene (BD) is a rodent carcinogen that is bioactivated to at least two genotoxic metabolites, 1,2-epoxybutene (EB) and 1,2,3,4-diepoxybutane (DEB). The mutational spectrum for DEB at hprt in human TK6 lymphoblasts (TK6 cells) was determined and compared with the mutational spectrum from s...
Topics
- Base Composition
- Carcinogens
- Cell Survival
- DNA Mutational Analysis
- DNA Transposable Elements
- Electrophoresis, Agar Gel
- Epoxy Compounds
- Exons
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Lymphocytes
- Mutagens
- Mutation
- Polymerase Chain Reaction
- RNA Splicing
- RNA, Messenger
- Sequence Deletion