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Article

Toward fast determination of protein stability maps: experimental and theoretical analysis of mutants of a Nocardiopsis prasina serine protease.

Biochemistry - 3 Jul 2012

Farrell Damien, Webb Helen, Johnston Michael A, Poulsen Thomas A, O'Meara Fergal, Christensen Lars L H, Beier Lars, Borchert Torben V, Nielsen Jens Erik

Abstract excerpt

The stability of serine proteases is of major importance for their application in industrial processes. Here we study the determinants of the stability of a Nocardiopsis prasina serine protease using fast residual activity assays, a feature classification algorithm, and structure-based energy calculation algorithms for 121 micropurified mutant enzyme clones containing multiple point mutations. Using a...

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PMID: 22668005
DOI: 10.1021/bi201926f

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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