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Article

Probing impact of active site residue mutations on stability and activity of Neisseria polysaccharea amylosucrase.

Protein science : a publication of the Protein Society - 1 Dec 2013

Daudé David, Topham Christopher M, Remaud-Siméon Magali, André Isabelle

Abstract excerpt

The amylosucrase from Neisseria polysaccharea is a transglucosidase from the GH13 family of glycoside-hydrolases that naturally catalyzes the synthesis of α-glucans from the widely available donor sucrose. Interestingly, natural molecular evolution has modeled a dense hydrogen bond network at subsite -1 responsible for the specific recognition of sucrose and conversely, it has loosened interactions at the subsite...

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Identifiers

PMID: 24115119
DOI: 10.1002/pro.2375
PMCID: PMC3843629

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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