Article
Cell mutants defective in synthesizing a heparan sulfate proteoglycan with regions of defined monosaccharide sequence.
Proceedings of the National Academy of Sciences of the United States of America - 1 Dec 1990
De Agostini A L, Lau H K, Leone C, Youssoufian H, Rosenberg R D
Abstract excerpt
We have demonstrated that mouse LTA cells synthesize cell-surface heparan sulfate proteoglycans (HSPGs) with regions of defined monosaccharide sequence that specifically interact with antithrombin (HSPGact). It remains unclear how HSPGact can be generated by a biosynthetic pathway with no simple template for directing the ordered assembly of monosaccharide units. To examine this issue, we treated LTA cells with...
Topics
- Animals
- Antithrombins
- Binding Sites
- Carbohydrate Sequence
- Cell Line
- Chondroitin Sulfate Proteoglycans
- Chromatography, Affinity
- Disaccharides
- Genetic Complementation Test
- Glycosaminoglycans