Article
Detection of single base mismatches of thymine and cytosine residues by potassium permanganate and hydroxylamine in the presence of tetralkylammonium salts.
Nucleic acids research - 11 Dec 1990
Gogos J A, Karayiorgou M, Aburatani H, Kafatos F C
Abstract excerpt
In the presence of tetramethylammonium chloride, potassium permanganate specifically modifies mismatched thymines. Similarly, the modification of mismatched cytosines by hydroxylamine was enhanced by tetraethylammonium chloride. Modification followed by piperidine cleavage permits specific identification of the T and C mismatches and by extension, when the opposite DNA strand is analyzed, of A and G mismatches as...
Topics
- Base Composition
- Base Sequence
- Cytosine
- DNA Mutational Analysis
- Female
- Humans
- Hydroxylamine
- Hydroxylamines
- Male
- Molecular Sequence Data
- Mutation