Article
Chemical cleavage of heteroduplex DNA to identify mutations.
Current protocols in human genetics - 1 May 2001
Cotton R G, Grompe M
Abstract excerpt
Mutation screening by the chemical-cleavage method is based on the fact that mismatched cytosine (C) and thymidine (T) are more reactive with the compounds hydroxylamine and osmium tetroxide, respectively, than are Watson and Crick-paired cytosine and thymidine bases. In this protocol, an excess of unlabeled target DNA is hybridized with labeled wild-type DNA probe and heteroduplexes are formed. One aliquot is...
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