Article
Mismatch oxidation assay: detection of DNA mutations using a standard UV/Vis microplate reader.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Tabone Tania, Sallmann Georgina, Cotton Richard G H
Abstract excerpt
Simple, low-cost mutation detection assays that are suitable for low-throughput analysis are essential for diagnostic applications where the causative mutation may be different in every family. The mismatch oxidation assay is a simple optical absorbance assay to detect nucleotide substitutions, insertions, and deletions in heteroduplex DNA. The method relies on detecting the oxidative modification products of...
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