Article
Complete mutation detection using unlabeled chemical cleavage.
Human mutation - 1 Jan 1992
Saleeba J A, Ramus S J, Cotton R G
Abstract excerpt
We have developed a strategy for the complete detection of point mutations, small insertions and deletions by chemical cleavage based on the methodology of Cotton et al. (1988). The technique was extended by the development of a nonisotopic cleavage product detection system using silver staining after gel electrophoresis. The complete mutation detection was achieved by use of mutant and wild-type DNAs in...
Topics
- Anemia, Sickle Cell
- Base Composition
- DNA
- DNA Transposable Elements
- Electrophoresis, Polyacrylamide Gel
- Genetics, Medical
- Globins
- Humans
- Mutation
- Point Mutation
- Sequence Deletion
- Silver