Detection of a single base mismatch in double-stranded DNA by electrophoresis on uncrosslinked polyacrylamide gel.

doi:10.1002/elps.11501501164

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Article

Detection of a single base mismatch in double-stranded DNA by electrophoresis on uncrosslinked polyacrylamide gel.

Electrophoresis - 1 Jan 2000

Pulyaeva H, Zakharov S F, Garner M M, Chrambach A

Abstract excerpt

Uncrosslinked polyacrylamide forms gels in the concentration range of 15-40% acrylamide. Electrophoresis in these gels of a commercially available 350 bp heteroduplex DNA preparation separates it from the homoduplex DNA of the same size. The separation is qualitatively equivalent to that previous...

Topics

Acrylic Resins
Base Composition
Cross-Linking Reagents
DNA
Electrophoresis, Polyacrylamide Gel
Gels
Genetic Techniques
Indicators and Reagents
Mutation
Nucleic Acid Heteroduplexes

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