Article
Mutation of potassium permanganate- and hydrogen peroxide-treated plasmid pZ189 replicating in CV-1 monkey kidney cells.
Mutation research - 1 Oct 1991
Akman S A, Forrest G P, Doroshow J H, Dizdaroglu M
Abstract excerpt
We examined the effect of the oxidation of plasmid pZ189 by KMnO4, which does not produce free radicals, and H2O2/Fe(2+)-diethylenetriaminepentaacetic acid (DTPA), which does, on the mutation frequency of pZ189 transfected into monkey kidney CV-1 cells. Treatment with 1.5 mM KMnO4 increased the content of certain modified bases, principally Thy and Cyt modified at C-5 and C-6, by up to 300-fold, as measured by...
Topics
- Animals
- Base Sequence
- Chlorocebus aethiops
- DNA Damage
- Hydrogen Peroxide
- In Vitro Techniques
- Molecular Sequence Data
- Mutation
- Oxidation-Reduction
- Plasmids
- Potassium Permanganate