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Article

Methylmethane-sulphonate and X-ray-induced mutations in the Chinese hamster hprt gene: mRNA phenotyping using polymerase chain reactions.

Mutagenesis - 1 Sept 1990

Chaudhry M A, Fox M

Abstract excerpt

Alterations in the hprt gene of Chinese hamster cells were determined in 71 spontaneous, methylmethane sulphonate (MMS)- and X-ray-induced mutants, using the Southern blot hybridization technique. Among 41 MMS-induced mutants, deletions eliminating the whole gene were observed in 17 cases (41%). Analysis of 20 X-ray-induced mutants revealed the presence of similar deletions in nine of them (45%). No evidence of...

Topics

Animals
Base Sequence
Blotting, Northern
Blotting, Southern
Cell Line
Exons
Genes
Hypoxanthine Phosphoribosyltransferase
Methyl Methanesulfonate
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Polymerase Chain Reaction

RNA, Messenger

Source

Identifiers

PMID: 2263207
DOI: 10.1093/mutage/5.5.497

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2263207-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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