Article
Rapid screening for deletion mutations in the hprt gene using the polymerase chain reaction: X-ray and alpha-particle mutant spectra.
Mutation research - 1 Sept 1992
Aghamohammadi S Z, Morris T, Stevens D L, Thacker J
Abstract excerpt
Conditions were devised for the isolation of DNA from single-mutant colonies on dishes, to give reproducible results in the polymerase chain reaction (PCR). Primers for 3 exons of the hamster hprt gene were used in a multiplex reaction to show rapidly whether the mutants carried deletions at these sites. 138 independent mutants were screened in total, some spontaneous and others induced by X-rays or by...
Topics
- Alpha Particles
- Animals
- Cell Line
- Chromosome Deletion
- Cricetinae
- DNA
- Hypoxanthine Phosphoribosyltransferase
- Mutagenicity Tests
- Mutation
- Polymerase Chain Reaction
- X-Rays